Camellia sinensis supplemented diet enhances the innate non-specific responses, haematological parameters and growth performance in Mugil cephalus against Photobacterium damselae

Shapour Kakoolaki; Paria Akbary; Mohamad Jalil Zorriehzahra; Hasan Salehi; Abolfazl Sepahdari; Mohamad Afsharnasab; Mohamad Reza Mehrabi; Salim Jadgal

doi:10.1016/j.fsi.2016.08.060

Camellia sinensis supplemented diet enhances the innate non-specific responses, haematological parameters and growth performance in Mugil cephalus against Photobacterium damselae

Fish Shellfish Immunol. 2016 Oct:57:379-385. doi: 10.1016/j.fsi.2016.08.060. Epub 2016 Aug 28.

Authors

Shapour Kakoolaki¹, Paria Akbary², Mohamad Jalil Zorriehzahra³, Hasan Salehi³, Abolfazl Sepahdari³, Mohamad Afsharnasab³, Mohamad Reza Mehrabi³, Salim Jadgal⁴

Affiliations

¹ Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Tehran, Iran. Electronic address: bsh443@gmail.com.
² Department of Marine Sciences, Chabahar Maritime University, Fisheries Group, Chabahar, Iran.
³ Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Tehran, Iran.
⁴ Offshore Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Chabahar, Iran.

PMID: 27582289
DOI: 10.1016/j.fsi.2016.08.060

Abstract

This study evaluated the effect of dietary supplementation of Camellia sinensis leaf-extract on non-specific immune responses and disease resistance of Mugil cephalus fingerling against P. damselae. Fish were fed with 0 (unsupplemented), 50, 100 and 200 mg/kg of green tea extract (GTE) supplemented diets. Results indicated that GTE decreased mortality in M. cephalus in a dose-dependent manner after challenge with P. damselae. Haematological parameters containing RBC, Hct, Hb and WBC and growth performance (weight gain) showed remarkable changes in comparison with control group. In addition, the phagocytic (PA) and respiratory burst activity (RBA) significantly increased in M. cephalus, fed 100 and 50, 100 and 200 mg/kg GTE, respectively. Lysozyme statistically increased in GTE supplemented fish. Overall, our results indicated that incorporation of C. sinensis supplemented diet at 100 and 200 mg/kg doses significantly enhanced the immune responses in M. cephalus and that the mortality percentage could be remarkably reduced after challenging the fish against P. damselae.

Keywords: Bacterium; Challenge; Dietary supplementation; Fish; Health; Immunity; Nutrition.

MeSH terms

Animal Feed / analysis
Animals
Camellia sinensis / chemistry*
Diet / veterinary
Dietary Supplements* / analysis
Disease Resistance
Dose-Response Relationship, Drug
Fish Diseases / immunology*
Fish Diseases / microbiology
Fishes*
Gram-Negative Bacterial Infections / immunology
Gram-Negative Bacterial Infections / microbiology
Gram-Negative Bacterial Infections / veterinary*
Immunity, Innate*
Photobacterium / physiology
Plant Extracts* / administration & dosage
Plant Leaves / chemistry
Random Allocation

Substances

Plant Extracts